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How to choose a suitable cell disruptor?
The following factors should be considered when choosing a suitable cell disruptor: Cell type: Different cell types (bacteria, yeast, plant, animal cells, etc.) are suitable for different disruption methods. Sample volume: When processing large sample volumes, a high-pressure homogenizer may be more suitable; when processing small sample volumes, an ultrasonic cell disruptor or a glass homogenizer may be more suitable. Cell concentration: High-concentration cell suspensions may require a high-pressure homogenizer or a bead mill. Processing mildness: If gentle processing is required to protect cell contents, choose a glass homogenizer or repeated freeze-thaw methods. Efficiency and throughput: When high-throughput processing is required, ultrasonic cell disruptors and high-pressure homogenizers are generally more effective.
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How does an ultrasonic cleaner work?
Ultrasonic cleaning machines use high-frequency ultrasonic vibrations (usually between 20kHz and 40kHz) to create tiny bubbles in the liquid, which are constantly formed and collapsed under pressure changes (called cavitation effect). The tiny explosions produced by this cavitation effect can effectively remove dirt, grease and other contaminants from the surface of items.
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What are the precautions when using an ultrasonic cell disruptor?
The following points should be noted when using an ultrasonic cell disruptor: Temperature control: Heat is generated during ultrasonic disruption, and the sample needs to be kept cool (such as an ice bath) to prevent overheating of the sample. Power and time settings: According to the cell type and volume, adjust the appropriate power and processing time to avoid excessive processing that may lead to degradation of cell contents. Cleaning and maintenance: Clean the probe before and after use to prevent cross contamination; check the equipment regularly to ensure normal operation. Avoid bubbles: Avoid bubbles in the sample during operation to ensure that ultrasound can be effectively transmitted to the cells.
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What are the precautions when using an ultrasonic cleaning machine?
When using an ultrasonic cleaning machine, pay attention to the following points: Cleaning fluid level: Make sure the cleaning fluid level is within the recommended range to ensure effective propagation of ultrasonic waves. Temperature control: Avoid temperatures that are too high or too low to prevent damage to the cleaning object or affect the cleaning effect. Cleaning time: Set an appropriate cleaning time based on the specific situation. Too long or too short may affect the effect. Regular maintenance: Clean the inner tank of the ultrasonic cleaning machine regularly and check the working status of the transducer. Safe operation: Avoid direct contact with the cleaning fluid and the inner tank of the cleaning machine during use to prevent injury or electric shock.
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How to maintain and service the ultrasonic cleaning machine?
Recommendations for maintaining and servicing ultrasonic cleaning machines include: Clean the inner tank regularly: prevent the accumulation of dirt and residual cleaning agent. Check the power supply and cables: ensure that the power connection and cables are intact. Drain the cleaning solution after cleaning: when not in use for a long time, drain the cleaning solution and wipe the inner tank dry. Check the transducer: regularly check the working status of the transducer to ensure its normal operation. Storage environment: place in a dry, ventilated environment, avoid moisture and corrosive gases.
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